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Grapevine Trunk Diseases Associated with Fungi from the Diaporthaceae Family in Croatian Vineyards (GTD)

J. Kaliterna, T. Milicevic, B. Cvjetkovic - Arh. Hig. Rada Toksikol. (2012) 63:471-479

Grapevine Trunk Diseases Associated with Fungi from the Diaporthaceae Family in Croatian Vineyards

Grapevine trunk diseases (GTD) have a variety of symptoms and causes. The latter include fungal species from the family Diaporthaceae. The aim of our study was to determine Diaporthaceae species present in the woody parts of grapevines sampled from 12 vine-growing coastal and continental areas of Croatia. The fungi were isolated from diseased wood, and cultures analysed for phenotype (morphology and pathogenicity) and DNA sequence (ITS1, 5.8S, ITS2). Most isolates were identified as Phomopsis viticola, followed by Diaporthe neotheicola and Diaporthe eres. This is the first report of Diaporthe eres as a pathogen on grapevine in the world, while for Diaporthe neotheicola this is the first report in Croatia. Pathogenicity trials confirmed Phomopsis viticola as a strong and Diaporthe neotheicola as a weak pathogen. Diaporthe eres turned out to be a moderate pathogen, which implies that the species could have a more important role in the aetiology of GTD.

Bolesti drva vinove loze povezane s gljivama iz porodice Diaporthaceae u hrvatskim vinogorjima

Bolesti drva vinove loze se oznacavaju kao grapevine trunk disease (GTD) i obuhvacaju nekoliko bolesti drva s razlicitom simptomatologijom i etiologijom. U skupinu uzrocnika bolesti drva pripadaju i fitopatogene gljive iz porodice Diaporthaceae. Do nedavno jeu Hrvatskoj  bila utvrdena samo vrsta Phomopsis viticola kao uzrocnik bolesti crna pjegavost i Phomopsis cotoneastri. U svijetu se navodi još 15 vrsta koje spadaju u rod Diaporthe/Phomopsis s razlicitom patogenosti na vinovoj lozi pa se stoga neke vrste smatraju endofitima, a neke patogenima.

Radi utvrdivanja etiologije bolesti drva vinove loze povezanih s gljivama iz porodice Diaporthaceae uzimani su uzorci bolesnog drva i rozgve vinove loze iz razlicitih vinogorja unutar svih 12 vinogradarskih podregija kontinentalne i primorske Hrvatske. Gljive su iz zaraženog drva izolirane u cistu kulturu na hranjivu podlogu. Taksonomski status izolata utvrden je na temelju njihove fenotipske karakterizacije (karakteristike kolonija i spora) i analizom DNA-sekvencija molekularnog markera ITS (ITS1, 5.8S, ITS2). Najveci broj izoliranih gljiva identificiran je kao vrsta Phomopsis viticola, dok je manji dio izoliranih gljiva pripadao vrstama Diaporthe neotheicola i Diaporthe eres. Za vrstu Diaporthe eres ovo je prvi nalaz na vinovoj lozi u svijetu, a za vrstu Diaporthe neotheicola prvi nalaz u Hrvatskoj. Testovi patogenosti potvrdili su da je Phomopsis viticola izrazito patogena vrsta na vinovoj lozi, dok se vrsta Diaporthe neotheicola pokazala slabim patogenom, a vrsta Diaporthe eres utvrdena je kao srednje jak patogen pa bi mogla imati važniju ulogu u etiologiji bolesti drva.

(Kristina Grozić)
Published on: 24/06/2016
European logoThis project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement N°652601
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